FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19077421
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT237850.022.8938755
CGAGTAG92900.020.0542583
TAGCTGG303800.019.1698387
GTAGCTG303550.018.5416876
GAGTAGC184900.018.2781034
CCGAGTA102900.017.8514752
CAAGTAG118350.017.0121783
AAAGTGC298450.016.7996884
AAGTAGC125800.016.1605914
CCAAAGT344350.016.155212
CTACTCG80650.015.8867442
AAGTGCT325000.015.5881945
GCTACTC176450.015.4561111
GCTGGGA767150.015.106269
CAAAGTG353850.015.0192713
GCTACTT124350.014.6738571
CCAAGTA145600.014.6365732
TTGGGAG424450.014.50777156
CTACTTG113200.014.4369932
TACTCGG90550.014.2940143
Produced by FastQC (version 0.11.5)