FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19038404
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT233800.022.7053345
CGAGTAG96400.020.7520923
CCGAGTA107150.019.2192252
GAGTAGC188050.018.9122434
TAGCTGG305100.018.6408167
GTAGCTG295300.018.2201826
AAAGTGC311700.017.6600864
CCAAAGT361500.016.710182
AAGTGCT337550.016.4431845
GCTACTC176600.015.8453751
CTACTCG87550.015.8305932
CAAAGTG371800.015.7725013
GCTGGGA785250.015.0098259
TACTCGG97550.014.8109363
CCCAAAG461250.014.7133111
TTGGGAG426350.014.5211186
CAAGTAG113950.014.1136163
GCTACTT120100.013.3374411
AAGTAGC121450.013.2958754
CTACTTG112150.013.2325672
Produced by FastQC (version 0.11.5)