FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18761042
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT226150.024.8177435
CGAGTAG90050.023.4865533
CCGAGTA98200.021.2013722
GAGTAGC182050.020.4667424
TAGCTGG298350.020.3733567
GTAGCTG288350.019.5561476
GCTACTC183750.018.0256791
CTACTCG88350.017.9331742
AAAGTGC318350.017.5662924
CCAAAGT364050.016.7970962
AAGTGCT343800.016.420245
TACTCGG102450.016.2977893
GCTGGGA773450.015.7276269
CAAAGTG381950.015.4289543
CTACTTG116100.015.2821852
GCTACTT125300.015.254581
CAAGTAG114750.015.2355823
TTGGGAG405250.015.1108436
AAGTAGC121050.014.4976344
CCCAAAG484600.014.2912661
Produced by FastQC (version 0.11.5)