FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19676414
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC300350.019.4706134
CCAAAGT355950.018.06452
CGAGTAG52750.017.9453743
AGTAGCT200150.016.956215
CAAAGTG375200.016.8945563
AAGTGCT350800.016.4505065
CTACTCG51800.016.3795242
GCTGGGA626300.016.198329
CCCAAAG452450.016.0285761
TAGCTGG240650.015.6741337
CCGAGTA62300.015.1946732
GCTACTC142500.014.8148281
GACTTGT156650.014.5476953
GAGTAGC139650.014.5110264
TACTCGG63000.014.1352793
CGAGACT82500.013.7722861
AGTGCTG442900.013.425466
ACTTGTG180650.013.3912894
GTAGCTG250300.013.1666736
TGATCAA180600.012.9661959
Produced by FastQC (version 0.11.5)