FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19564966
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC300000.019.4361884
CCAAAGT355950.018.325482
AAGTGCT341650.016.9038625
CAAAGTG373600.016.6008743
AGTAGCT193850.016.450755
CCCAAAG448550.016.4486371
GCTGGGA604850.016.204159
CTACTCG53200.016.0672972
TAGCTGG232700.015.1826117
CGAGTAG53650.015.0218253
TACTCGG65100.014.5327363
CCGAGTA61400.014.4920562
AGTGCTG437450.013.5877156
GCTACTC143900.013.0979671
GTAGCTG248100.013.0807696
GAGTAGC137350.013.0112954
CTACTTG131500.012.094692
GCTAATT151600.011.8780821
TTGGGAG352400.011.87455756
GCTACTT140850.011.8394771
Produced by FastQC (version 0.11.5)