FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19286838
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC300950.018.8698624
CGAGTAG56050.018.512223
CCAAAGT354250.017.7918972
AGTAGCT200900.017.3790635
AAGTGCT342650.016.8393765
TAGCTGG234100.016.3818457
CAAAGTG371000.016.2705293
CCCAAAG448200.016.160171
GAGTAGC137150.016.1535994
GCTGGGA621250.016.0364829
CCGAGTA63850.015.9213362
CTACTCG51350.015.701092
GCTACTC139050.014.3711431
GTAGCTG244000.013.9357026
TACTCGG61450.013.8049833
GACTTGT156150.013.7837943
CGAGACT89300.013.6620291
AGTGCTG436300.013.289156
ACTTGTG179500.012.5377214
TGATCAA174950.012.5032269
Produced by FastQC (version 0.11.5)