FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19660267
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC296500.019.2732564
CCAAAGT351500.018.0036852
CCCAAAG444100.016.6403311
AAGTGCT347300.016.535975
CAAAGTG366950.016.274183
CTACTCG53050.016.098772
GCTGGGA614000.015.23671159
AGTAGCT194250.015.1789495
CGAGTAG49400.015.0222833
TAGCTGG227350.014.5134797
TACTCGG63400.014.2448353
CCTATAT77800.013.8653681
GCTACTC139650.013.6934211
AGTGCTG442150.013.3865456
CCGAGTA58550.012.7930822
GAGTAGC135500.012.40399554
GTAGCTG242700.012.3813776
GCTATAT75600.012.230451
GCTAATT149750.011.9278911
TTGGGAG353100.011.8428996
Produced by FastQC (version 0.11.5)