FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18683220
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC274150.019.2077754
CCAAAGT334250.017.53442
AAGTGCT319500.016.795555
CCCAAAG417450.016.416971
CAAAGTG351000.016.1409423
CGAGTAG47950.015.6854173
GCTGGGA579300.015.5475189
AGTAGCT181850.015.315195
TAGCTGG209100.015.3079347
CTACTCG46500.015.1127442
CCGAGTA58400.014.6805822
AGTGCTG410850.013.5936446
GCTACTC128000.013.2891811
TACTCGG58800.012.7910853
GTAGCTG226100.012.4750566
GAGTAGC128100.012.18392854
GTGCTGG553100.011.409017
TTGGGAG323750.011.037046
TGCTGGG634200.010.9045128
GCTAATT144450.010.8512581
Produced by FastQC (version 0.11.5)