FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19957902
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC304300.018.5546234
CCAAAGT361450.017.395552
CGAGTAG57000.016.694733
CGTCGAG31350.016.638561
AAGTGCT354150.016.1816885
CAAAGTG383500.015.9174333
AGTAGCT197350.015.8943725
CCCAAAG457650.015.7566451
GCTGGGA630250.015.713869
CCGAGTA64500.015.4092312
CTACTCG52600.015.0090722
TAGCTGG238450.014.8693967
GAGTAGC138650.014.1333954
CGAGACT92050.013.8604181
AGTGCTG445400.013.3887686
GCTACTC144950.013.0814431
GTAGCTG249900.012.9469336
TACTCGG65300.012.8455953
GACTTGT157750.012.6008613
GTCGAGA44950.012.5453241
Produced by FastQC (version 0.11.5)