FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18994472
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC278100.018.9875774
CCAAAGT345000.017.060732
CGAGTAG53100.016.7064933
AAGTGCT321150.016.6395235
CCCAAAG430050.016.4862161
CAAAGTG357450.015.7967723
CTACTCG50450.015.7698552
CCGAGTA61150.015.19805052
TAGCTGG222650.015.1737487
GCTGGGA600200.014.9048019
AGTAGCT190300.014.6878665
GCTACTC129800.014.0486781
GACTTGT153950.013.8113623
GAGTAGC136200.013.543584
TACTCGG61750.013.4540893
GTCGAGA38900.013.3934531
AGTGCTG411850.013.299916
CGAGACT84950.013.1778371
ACTTGTG180050.012.4349284
GTAGCTG235850.012.2392866
Produced by FastQC (version 0.11.5)