FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18994472
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC283700.018.9343244
CCAAAGT334800.017.3229772
CTACTCG52550.016.8558942
CGAGTAG49600.016.7256473
AAGTGCT319600.016.5907965
GCTGGGA586450.016.1004549
AGTAGCT179550.015.9800245
CAAAGTG353450.015.7734913
CCCAAAG429650.015.4631271
TACTCGG60250.015.1694163
CCGAGTA59800.015.0474462
TAGCTGG217600.014.7087737
GCTACTC134550.014.6303471
GAGTAGC127750.014.3096244
AGTGCTG410250.013.4584746
GTAGCTG231950.012.3113726
GCTATAT71950.012.2140461
TTGGGAG330550.011.4264646
CCCGAGT104200.011.2675391
CTGTCTA138700.011.121931145
Produced by FastQC (version 0.11.5)