FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18901050
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC275250.018.8129864
CTACTCG50950.018.4729252
CCAAAGT333050.017.145742
CCCAAAG422500.016.2480181
AAGTGCT320950.016.183565
CAAAGTG350150.015.5880353
TACTCGG62650.014.9126593
GCTGGGA582700.014.9064089
TAGCTGG215650.014.6712827
AGTAGCT183400.014.4863675
GCTACTC135050.014.1516421
GCTAATT143200.013.1499561
AGTGCTG410650.012.8221866
CGAGTAG47150.012.8127173
GAGTAGC128600.012.5662144
GTAGCTG228000.011.9324126
CCTATAT77850.011.8234771
GGAGGCT459250.011.516489
GCTATAT69100.011.3886571
CTGTCTA138500.011.169494145
Produced by FastQC (version 0.11.5)