FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19399305
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC269000.017.619864
CCAAAGT323600.015.8184262
AAGTGCT319950.015.274895
CAAAGTG343950.014.6989743
CCCAAAG424050.014.3895421
CGAGTAG45050.014.0280583
GCTGGGA577700.012.9911599
TAGCTGG204950.012.81152257
CCTATAT68150.012.5700861
GCTAATT125200.012.4506751
GTCGAGA38600.012.006071
AGTAGCT171200.011.9354895
AGTGCTG423450.011.5414496
GAGTAGC118250.011.282454
CCGAGTA58150.010.9884372
CTACTCG48900.010.913122
GCTATAT65650.010.8026641
GCTACTC122300.010.7364181
GACTTGT146250.09.8425543
GTAGCTG228600.09.7065326
Produced by FastQC (version 0.11.5)