FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19891443
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC269900.016.53464
CCAAAGT335350.014.9837572
AAGTGCT323300.013.825295
CCCAAAG430150.013.7925541
CAAAGTG349250.013.7034273
CGAGTAG48650.013.5788653
TAGCTGG199750.012.7001037
AGTAGCT173300.012.5714685
GCTGGGA589100.012.4654389
CTACTCG46150.012.1823572
GCTACTC131450.011.818271
CCTATAT70850.011.6082891
GCTAATT130700.011.5633871
GCTATAT70100.011.3313751
TACTCGG60200.011.0903593
GATATAT97800.010.9251381
CCGAGTA57100.010.8307752
AGTGCTG433000.010.6959756
GAGTAGC123600.010.519044
GCTACTT136250.010.00892451
Produced by FastQC (version 0.11.5)