FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20014974
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC270100.017.272424
CCAAAGT332600.015.8588672
CCCAAAG434850.014.8421131
CAAAGTG348400.014.6984363
CTACTCG49600.014.4435172
AAGTGCT327100.014.2644785
GCTGGGA588600.013.2557949
AGTAGCT172100.013.2291685
TAGCTGG199550.013.2077997
CGAGTAG46350.012.7306713
TACTCGG58150.012.3217453
CCTATAT73100.011.8170051
GCTATAT72250.011.7616231
GCTAATT129300.011.3518721
GCTACTC138800.011.1820781
AGTGCTG439000.011.1419136
CCGAGTA57150.011.0606522
CCTAATA76000.010.3496141
GTAGCTG228050.010.2619326
GAGTAGC124800.09.7396034
Produced by FastQC (version 0.11.5)