FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19343896
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC268050.017.9317554
CCAAAGT319950.016.6262822
AAGTGCT324550.015.2431085
CAAAGTG345850.014.8934893
CCCAAAG412000.014.7371171
CGAGTAG47400.014.2322363
GCTACTC121750.014.0259811
GCTGGGA579750.013.8791169
TAGCTGG201900.013.1915057
CTACTCG47550.012.5617392
AGTAGCT172250.012.5245365
AGTGCTG418150.012.2345016
GCTAATT127550.011.62513351
GAGTAGC126250.011.2435254
CGTCGAG33100.011.0400571
CCGAGTA60550.010.9092552
GCTATAT65350.010.3233741
GATATAT96750.010.3141481
TACTCGG60250.010.2637463
GTGCTGG572700.09.9514237
Produced by FastQC (version 0.11.5)