FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19891443
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC275950.016.8775124
CCAAAGT332800.015.805812
CCCAAAG426500.014.5709121
CAAAGTG347200.014.5249393
AAGTGCT322150.014.4147055
GCTGGGA588450.013.3986669
GCTATAT69300.012.463341
CGAGTAG46200.011.8582943
CCTATAT73500.011.8466851
AGTAGCT169950.011.6570975
TAGCTGG197750.011.4769687
GCTAATT133000.011.4041961
AGTGCTG428900.011.13936
CTACTCG50500.010.7082272
GCTACTC137100.010.1412221
GATATAT101900.010.0610011
CCGAGTA58350.09.8694112
GGAGGCT464600.09.6649589
GCTAATA69400.09.5111041
GCTATTA76550.09.4483191
Produced by FastQC (version 0.11.5)