FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20386692
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG49200.018.3359472
AAAGTGC293650.017.34124
CCAAAGT352150.016.3042
AAGTGCT342150.014.9694045
CAAAGTG369200.014.7193323
CGAGTAG48350.014.6101163
CCCAAAG452450.014.5159391
GCTACTC136450.014.4552531
TACTCGG62400.013.4500983
GCTGGGA617550.013.0659419
CTGTCTA146650.012.564553145
AGTAGCT180150.012.1956615
CCGAGTA59700.012.1825372
AGTGCTG448100.011.8524096
GCTATAT72000.011.8516131
TAGCTGG218400.011.75888257
GCTACTT140600.011.3422611
GCTAATT142900.011.3065431
CCTATAT77150.011.0604811
GGAGGCT471800.010.5758469
Produced by FastQC (version 0.11.5)