FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20346243
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC295950.017.2130154
CCAAAGT349450.016.1915572
AAGTGCT345000.015.055715
CAAAGTG372700.014.7347433
CCCAAAG455650.014.7196291
CTACTCG49300.014.5941562
CGAGTAG49200.014.0588413
GCTGGGA616750.013.7414939
CTGTCTA146250.013.521545145
TAGCTGG217800.013.0340637
AGTAGCT185650.012.6901415
TACTCGG60900.012.5051423
GCTACTC135350.012.181781
AGTGCTG450200.011.8340776
GCTATAT70200.011.5445741
GCTAATT139950.011.4319311
CCGAGTA62350.011.2034622
GAGTAGC128900.011.0050214
GCTATTA81650.010.267911
GGAGGCT465900.010.0559389
Produced by FastQC (version 0.11.5)