FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20184728
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC287350.016.5591344
CCAAAGT355200.015.5827652
CTACTCG48800.014.3390742
CAAAGTG373100.014.2349663
CCCAAAG448050.014.1032571
AAGTGCT341150.013.906675
GCTGGGA610450.013.4801919
AGTAGCT178750.013.4663915
CGAGTAG48800.013.0485263
TAGCTGG213400.012.7553987
GCTAATT142150.012.1592911
GCTACTC132400.011.83908751
GCTATAT72750.011.3502961
GAGTAGC123100.011.3118834
TACTCGG61400.011.28253653
CCGAGTA59050.011.2575712
AGTGCTG443100.011.1965276
CCTATAT74350.011.0119211
GTCGAGA44900.010.2862191
CTACTTG129200.010.18207552
Produced by FastQC (version 0.11.5)