FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20386692
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC288300.017.5969434
CGAGTAG48650.017.1162643
CTACTCG50150.015.7671212
CCAAAGT357750.015.0610752
AAGTGCT343700.014.7605425
AGTAGCT180700.014.4055085
CAAAGTG373500.014.2573543
GCTGGGA624550.014.1620059
CCCAAAG456850.014.0085921
TAGCTGG220100.013.6390217
CCGAGTA60750.013.47672752
GCTACTC137650.012.5644251
TACTCGG62350.012.4575143
GAGTAGC133850.012.3377914
AGTGCTG446150.012.0298256
GTAGCTG240600.011.0227126
GCTAATT149800.010.8909561
GCTATAT72700.010.8834341
CTACTTG133700.010.4675122
GCTACTT143050.010.3280061
Produced by FastQC (version 0.11.5)