FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20106256
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG56950.021.99333
AAAGTGC340500.021.4337584
CCAAAGT411600.019.5673072
GCTGGGA637500.019.0528839
AAGTGCT382900.018.8958445
CCGAGTA69350.018.8680172
AGTAGCT232100.018.72185
CTACTCG55250.018.490632
CAAAGTG423400.018.3609143
CCCAAAG495150.018.0337071
GCTACTC152500.017.9422951
TAGCTGG257850.017.935957
GAGTAGC154250.016.9659254
GTAGCTG257350.016.721936
AGTGCTG456700.015.8885116
TACTCGG64450.015.4168813
GGAGGCT480150.014.1346799
CCCGAGT109450.014.0662381
GTGCTGG566500.013.832747
TGCTGGG645750.013.0452758
Produced by FastQC (version 0.11.5)