FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20112955
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC331250.019.8999654
CGAGTAG52050.019.3595283
CCAAAGT393150.018.9204852
CCCAAAG468700.017.9484631
AAGTGCT371900.017.9141035
AGTAGCT222450.017.8695
GCTGGGA634050.017.7860039
CTACTCG55300.017.7157782
CAAAGTG408600.017.417093
CCGAGTA57850.017.2977642
TAGCTGG267350.016.4937827
GAGTAGC149550.016.4239654
GCTACTC158750.015.8269951
AGTGCTG438500.015.354066
TACTCGG63400.015.1211323
GTAGCTG264550.014.9471526
TTGGGAG377950.013.7029856
GGAGGCT467600.013.4733119
GTGCTGG555850.012.6677727
CTACTTG145100.012.4907832
Produced by FastQC (version 0.11.5)