FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20112955
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG54650.020.8741953
AAAGTGC331950.020.4930154
CCGAGTA61000.019.848542
CCAAAGT398900.018.7553842
CTACTCG56950.018.6793652
GCTGGGA630650.018.3213279
AGTAGCT224350.018.1867355
AAGTGCT382550.017.8555775
CAAAGTG414300.017.517663
CCCAAAG483800.017.3239481
TAGCTGG262000.017.2553187
TACTCGG64200.017.224013
GAGTAGC157050.016.0426774
GCTACTC156350.015.8534551
AGTGCTG454350.015.1726426
GTAGCTG267100.015.1188046
GGAGGCT479100.013.7163229
TTGGGAG373500.013.5288886
CCCGAGT103750.013.4302321
GCTACTT158500.012.7669491
Produced by FastQC (version 0.11.5)