FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20193580
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG57600.021.267862
AAAGTGC334750.020.6839984
CGAGTAG54450.019.6711483
CCAAAGT394900.019.4458872
TACTCGG65300.019.0828423
AAGTGCT381350.018.5427745
GCTGGGA629350.018.3249579
CAAAGTG413050.018.2027853
AGTAGCT220900.018.002375
CCCAAAG476800.017.8397661
GCTACTC161200.017.41521
CCGAGTA62150.017.3455412
TAGCTGG256850.016.9287827
AGTGCTG449200.015.9609936
GAGTAGC152250.015.9102724
GTAGCTG257000.015.0657826
GGAGGCT473500.014.1081489
GTGCTGG565700.013.4046637
CCCGAGT101250.013.1373471
TTGGGAG382050.012.9568346
Produced by FastQC (version 0.11.5)