FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20139592
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG59400.021.5542982
CGAGTAG53600.021.4065253
AAAGTGC338500.020.3791164
CCGAGTA62150.019.137142
TACTCGG66000.019.080763
CCAAAGT411650.019.0352752
AGTAGCT229450.018.7522815
GCTGGGA643300.018.6952639
AAGTGCT388400.018.3012855
TAGCTGG262800.018.2893587
GCTACTC161050.018.0789551
CCCAAAG494450.017.8073391
CAAAGTG428250.017.5948453
GAGTAGC154500.016.2567254
GTAGCTG267950.015.9534466
AGTGCTG453150.015.454656
GGAGGCT467200.014.121419
CCCGAGT106750.014.0309341
GCTACTT160200.013.4564011
GTGCTGG572150.013.1084697
Produced by FastQC (version 0.11.5)