FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20139592
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG59900.021.139882
CGAGTAG56950.021.0077763
AAAGTGC334900.020.3085294
CCGAGTA63300.019.009712
TACTCGG65400.018.7213883
CCAAAGT395300.018.6005882
AGTAGCT223200.018.4353085
GCTACTC158950.018.3664041
GCTGGGA626650.018.1184259
AAGTGCT375000.018.0079125
CAAAGTG411450.017.3614673
CCCAAAG477900.017.1394711
TAGCTGG258350.017.0947047
GAGTAGC153500.016.820754
GTAGCTG265350.015.8248816
AGTGCTG447400.015.0952256
GGAGGCT477500.014.8061679
TTGGGAG383400.014.055526
CTACTTG146300.013.9633392
CCCGAGT103550.013.7244361
Produced by FastQC (version 0.11.5)