FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20193580
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG58850.020.2236612
AAAGTGC342100.020.0142774
CGAGTAG55700.019.1048953
CCAAAGT409500.018.6350232
AGTAGCT231150.018.3238935
GCTGGGA631950.018.2348549
GCTACTC160200.017.9721551
TACTCGG66050.017.9130743
AAGTGCT389500.017.6146835
CAAAGTG424100.017.4321883
CCCAAAG489200.017.3841361
TAGCTGG264950.017.1225057
GAGTAGC154950.016.2654114
CCGAGTA66950.016.1037672
GTAGCTG264100.015.9051996
AGTGCTG461000.014.8523176
TTGGGAG371550.014.2072616
GGAGGCT476000.014.1195049
GCTACTT157500.013.5211591
CTACTTG151850.013.3241772
Produced by FastQC (version 0.11.5)