FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20125019
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG61750.025.3316153
CCGAGTA67600.023.5786842
AAAGTGC357300.021.7090664
CTACTCG66200.021.5428712
AGTAGCT257300.020.7286785
GCTGGGA680400.020.1897189
GAGTAGC168200.019.7994214
CCAAAGT421950.019.6662312
TACTCGG75100.019.3408363
TAGCTGG311700.019.1249497
AAGTGCT399350.019.0891675
CCCAAAG502850.019.042361
CAAAGTG436000.018.7231923
GTAGCTG288250.018.6224696
GCTACTC186250.016.9117831
AGTGCTG469500.016.4437086
TTGGGAG422200.016.2523276
CCCGAGT110700.016.0605071
GGAGGCT515350.014.8990949
GTGCTGG574700.014.0287827
Produced by FastQC (version 0.11.5)