FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19986356
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG65150.022.6079733
CCGAGTA70900.022.349782
AGTAGCT264700.021.0974355
AAAGTGC357700.020.82284
CTACTCG65700.020.0812172
CCAAAGT422700.019.9163022
GCTGGGA689400.019.8642149
TACTCGG72050.019.4740223
TAGCTGG305500.019.442657
GTAGCTG292300.018.9620516
AAGTGCT397150.018.8598735
GAGTAGC176650.018.809924
CCCAAAG508900.018.6826421
CAAAGTG438750.018.3763163
GCTACTC174300.016.6605571
AGTGCTG464450.016.2923566
CCCGAGT118350.015.3355271
GGAGGCT508750.014.9954459
TTGGGAG424350.014.7557696
GTGCTGG572200.014.14977
Produced by FastQC (version 0.11.5)