FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19986356
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC358300.021.1399674
CCGAGTA69200.021.118452
CGAGTAG65550.021.096883
AGTAGCT256600.020.4276185
CCAAAGT431000.019.5493182
GCTGGGA670200.019.4837389
AAGTGCT391900.019.25245
GAGTAGC167200.018.7469844
CTACTCG67600.018.7220652
CCCAAAG504700.018.5730061
TAGCTGG302950.018.2917697
CAAAGTG444300.018.109313
GTAGCTG286450.017.956636
GCTACTC178050.017.5752051
TTGGGAG423750.016.684226
CCCGAGT111050.016.6425631
AGTGCTG462250.016.5477836
TACTCGG73800.016.0886023
GGAGGCT509950.015.1093029
GTGCTGG570350.014.0966927
Produced by FastQC (version 0.11.5)