FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18866819
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC279000.020.3755254
CTACTCG57700.018.941982
CCAAAGT343700.018.873272
AAGTGCT325500.017.682775
CAAAGTG354900.017.21783
GCTACTC137500.016.9843331
CGAGTAG48250.016.915353
TACTCGG65950.016.8952773
CCCAAAG445050.016.3163171
AGTAGCT177650.015.5005465
GCTGGGA626300.015.3087449
TAGCTGG216200.014.7716197
CGTCGAG28000.014.19663051
AGTGCTG435300.013.8094856
CCGAGTA60400.013.7476812
GAGTAGC130350.013.0671454
CCTATAT69900.012.6936961
GTAGCTG232700.012.291126
GCTATAT67850.011.6125721
GCTAATT139450.011.5548061
Produced by FastQC (version 0.11.5)