FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19327098
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC289450.021.0454274
CCAAAGT347150.018.7745022
CGAGTAG49300.018.7214453
CAAAGTG358000.017.8286953
AAGTGCT345550.017.7110275
CCCAAAG446350.016.860611
AGTAGCT180400.016.7262955
CTACTCG51650.016.082682
TAGCTGG218800.015.8991827
GCTGGGA653800.015.8322689
GCTACTC132400.015.6580531
CCGAGTA60900.015.2720342
GAGTAGC133200.014.178214
AGTGCTG444800.014.1103666
TACTCGG63650.013.9429453
GTAGCTG239000.012.5955966
GTCGAGA39250.012.4810851
CCTATAT75200.012.3679111
GCTACTT138750.012.2806431
CTACTTG133750.012.1027482
Produced by FastQC (version 0.11.5)