FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20367927
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC293350.018.3731584
CCAAAGT347950.017.5035232
AAGTGCT337100.016.0297815
CTACTCG52600.015.956862
CAAAGTG368100.015.7350633
CCCAAAG451750.015.3242471
GCTGGGA615900.014.8786629
AGTAGCT190300.014.872035
CGAGTAG50750.014.078263
TAGCTGG227300.014.0380667
GCTACTC140350.012.9984051
TACTCGG64550.012.6804183
AGTGCTG443050.012.6348376
CCGAGTA62900.012.3513722
GAGTAGC138100.012.2056664
GTAGCTG252400.011.267916
GCTACTT138450.011.072511
TTGGGAG341750.011.0620826
GGAGGCT476400.011.037459
CCTATAT75550.010.7423221
Produced by FastQC (version 0.11.5)