FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20565740
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC293450.018.5693994
CCAAAGT362750.016.6996382
CGTCGAG34650.016.5594371
AAGTGCT338500.016.1604775
ACGTCGA24250.015.8703291
CAAAGTG376950.015.5879873
CCCAAAG462000.014.9337851
CTACTCG50350.014.7266162
GCTGGGA614200.014.726279
TAGCTGG226000.013.5572567
CGAGTAG49900.013.177163
AGTAGCT193200.013.1433365
GCTACTC137000.012.8200031
AGTGCTG442400.012.8078436
GTCGAGA48100.012.216052
TACTCGG61600.012.0370383
CCTATAT78650.011.5659041
CCGAGTA59900.011.2109142
GCTATAT77650.010.7235961
GAGTAGC131250.010.712514
Produced by FastQC (version 0.11.5)