FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20565740
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC298050.016.8054774
CCAAAGT358650.016.2241952
CGAGTAG49250.014.9126953
CCCAAAG456500.014.799321
AAGTGCT344750.014.7944525
CAAAGTG374300.014.6136933
GCTGGGA611400.014.5959629
AGTAGCT186950.014.3708135
CTACTCG53650.014.3394892
TAGCTGG224650.014.2368327
GCTACTC137800.013.7031361
CCGAGTA59400.013.3046282
AGTGCTG440100.012.2268646
TACTCGG66050.011.9668183
GCTATAT77800.011.8658751
GAGTAGC131750.011.7877074
GCTAATT149400.011.5625011
GTAGCTG245600.011.0546116
GGAGGCT475250.010.3680839
GTGCTGG596000.010.2511257
Produced by FastQC (version 0.11.5)