FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20927456
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC293700.018.7035584
CCAAAGT360750.016.698822
AAGTGCT346000.016.1606315
CCCAAAG458900.015.5365461
CAAAGTG381600.015.1645573
GCTGGGA613200.014.3501299
CTACTCG58450.013.2870152
AGTGCTG444450.013.1176626
CCTATAT82900.012.4068381
TAGCTGG225350.012.1453397
AGTAGCT190650.011.7462925
TACTCGG67200.011.5580963
GCTACTC144350.011.3906741
TTGGGAG354200.011.1446116
GTGCTGG591900.010.927337
CGAGTAG50350.010.2840653
GCTATAT77100.010.2546861
GGAGGCT478700.010.1927659
GCTATAG72350.010.0575711
TGCTGGG687450.010.0503468
Produced by FastQC (version 0.11.5)