FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20927456
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC294350.019.6830944
ACGTCGA25150.018.0915051
CCAAAGT364250.017.1929132
AAGTGCT351750.016.5316725
CAAAGTG379300.016.160263
CCCAAAG460250.015.8023271
CGAGTAG53250.015.6370333
GCTGGGA626350.015.1158169
AGTAGCT206100.014.1921495
GCTACTC140650.014.1841871
TAGCTGG232500.014.1759427
CTACTCG52500.013.9944552
CGTCGAG33500.013.7855832
CCGAGTA62400.013.6804762
AGTGCTG443850.013.4166226
GAGTAGC140600.012.4417574
GTAGCTG257550.011.4385356
TACTCGG65550.011.4218933
GCTAATT152500.011.0623271
CCTATAT78800.010.7487631
Produced by FastQC (version 0.11.5)