FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17229666
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC249000.018.3161744
CCAAAGT299550.017.1384562
CTACTCG49100.016.5467822
AAGTGCT285650.016.1888435
CAAAGTG318050.015.5050653
CCCAAAG388400.015.4718621
TAGCTGG195550.014.3002487
GCTACTC120050.014.1768131
CGAGTAG44250.014.0887693
GCTGGGA535300.014.0635479
AGTAGCT166450.013.7648045
TACTCGG57050.013.5062063
CCGAGTA50900.013.3472252
AGTGCTG379550.012.8505236
GAGTAGC117400.011.6364264
CCTATAT63900.011.3990071
GTAGCTG212150.011.2970136
GCTAATT128950.011.0257771
GGAGGCT419100.010.8379019
GCTATAT63800.010.5386521
Produced by FastQC (version 0.11.5)