FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18185218
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC270550.017.6815074
CCAAAGT322200.016.4775772
AAGTGCT311400.015.6769455
CCCAAAG405150.015.5447681
CAAAGTG335400.014.9101233
GCTGGGA560800.014.6873359
AGTAGCT175700.014.6697695
CGAGTAG47700.014.0960993
TAGCTGG206250.014.02497
CTACTCG47850.013.7592752
CCGAGTA57700.013.7167842
GCTACTC129700.013.2332511
CGTCGAG30150.012.5472011
AGTGCTG396400.012.50976
ACGTCGA19600.012.1524331
GAGTAGC122650.011.7066434
CCTATAT70300.011.4599561
GCTAATT142650.011.44259551
GTAGCTG226850.011.2694156
TACTCGG58350.011.1631873
Produced by FastQC (version 0.11.5)