FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17229666
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG46250.017.4198442
AAAGTGC247350.017.2489344
CGAGTAG48300.017.1155853
ACGTCGA22700.015.7445331
CCAAAGT299150.015.362892
CCGAGTA53550.015.1759772
AGTAGCT166400.015.1571455
CCCAAAG384250.014.9184891
AAGTGCT294750.014.689365
TACTCGG52450.014.292073
GCTGGGA534750.014.2280289
CAAAGTG317000.014.1883743
TAGCTGG200700.014.1724737
CGTCGAG29600.013.0175462
GCTACTC125100.012.8281371
GTAGCTG213750.012.2583976
GAGTAGC119150.012.2299924
AGTGCTG375250.011.7061776
GCTACTT122750.011.075561
GCTAATT133750.011.0553911
Produced by FastQC (version 0.11.5)