FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17502866
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC259850.017.5786574
CCAAAGT308700.016.9505562
CGAGTAG43300.015.6929163
CCCAAAG392600.015.5765091
AAGTGCT301450.015.2931695
CAAAGTG324700.015.1883363
CTACTCG50600.015.0895392
GCTGGGA537600.014.1709039
AGTAGCT167750.013.9916565
TACTCGG58950.013.4280983
GCTACTC125800.013.196961
TAGCTGG200800.012.7736437
GAGTAGC118650.012.5768744
AGTGCTG381600.012.284486
CCGAGTA52500.011.874922
GTAGCTG212000.011.30392556
GCTATAT65800.011.0716831
CCTATAT69250.011.0258711
GGAGGCT421450.011.0087789
TTGGGAG306900.010.9593136
Produced by FastQC (version 0.11.5)