FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20291113
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC268950.018.089454
CCAAAGT330700.015.9723842
AAGTGCT322750.015.5910945
CAAAGTG344100.015.1890273
CCCAAAG439000.014.5655371
GCTGGGA609300.013.8656339
AGTAGCT168850.012.4311165
AGTGCTG442900.012.1618686
CGAGTAG50800.012.0397013
TAGCTGG204800.011.7039037
CGTCGAG32800.011.2306591
CCTATAT72400.011.1358311
GCTACTC129700.011.0926051
GTCGAGA43900.010.9241521
CTACTCG49050.010.7687412
CCGAGTA62700.010.7521312
GCTATAT66700.010.7328391
GAGTAGC124200.09.7929924
GCTAATT121950.09.6318141
GTGCTGG614350.09.5109017
Produced by FastQC (version 0.11.5)