FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20291113
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC267100.017.0891344
CCAAAGT323950.015.4412742
AAGTGCT312000.015.0751875
CAAAGTG341900.014.264763
CCCAAAG434600.014.2137661
GCTGGGA604000.012.8949219
CGAGTAG50050.011.7988953
TAGCTGG200650.011.7379537
AGTGCTG434150.011.6020386
CTGTCTA136950.011.5569725145
AGTAGCT165350.011.5126415
GCTATAT65700.011.2108271
CCTATAT68900.011.1944021
GCTAATT117950.011.1931621
GCTATTA77400.010.9525691
CTACTCG51750.010.7400982
CCGAGTA59750.010.5811312
GCTACTC126150.010.4655841
CCTAAAT110400.010.3221911
GCTATAG64450.010.0266791
Produced by FastQC (version 0.11.5)