FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815690
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC260450.018.1693944
CCAAAGT317850.016.4844512
AAGTGCT305650.015.3461055
CCCAAAG421600.014.9685461
CAAAGTG336100.014.9274753
GCTATAT64100.013.9870471
ACGTCGA26350.013.1881471
GCTGGGA590600.012.5068099
CGTCGAG37100.011.9894841
CTACTCG45850.011.9732792
AGTGCTG426100.011.8068716
GCTAATT114750.011.0839051
GCTACTC126350.010.8914161
CCTATAT69400.010.7156551
GCTAATA62350.010.5896481
GTCGAGA45550.010.2230571
TACTCGG60750.010.180323
TAGCTGG201650.010.1654647
CTATATG65750.010.1461392
AGTAGCT170150.09.9649855
Produced by FastQC (version 0.11.5)