FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20036899
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC258300.018.6267594
CCAAAGT322000.016.9513422
CAAAGTG340350.015.54612353
AAGTGCT310300.015.4380325
CTGTCTA135800.015.117954145
CCCAAAG435400.014.7885231
CTACTCG52350.014.0792472
GCTGGGA597300.013.2808199
AGTGCTG420200.012.111376
TACTCGG63350.011.524073
GCTACTC130800.011.482811
GCTAATT115400.011.4485361
TAGCTGG198300.011.1492567
AGTAGCT162700.010.8970045
CCTATAT66600.010.440661
CGAGTAG44000.010.4292823
GCTATAT65400.09.8879761
CCTAATA71800.09.8782031
GCTATAA80550.09.7547261
TGCTGGG687150.09.7436258
Produced by FastQC (version 0.11.5)