FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19712313
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC256250.017.2674754
CCAAAGT317000.015.4485052
CAAAGTG332200.014.2188973
AAGTGCT315050.014.1109425
CCCAAAG428300.013.5098751
GCTGGGA599050.012.7371099
AGTAGCT165650.012.7059365
TAGCTGG196950.012.3381957
CGAGTAG46800.012.1709543
CTACTCG47100.012.0934322
CCGAGTA56550.011.9150462
CCTATAT69250.011.5353961
GCTACTC124350.011.1721971
AGTGCTG421300.010.6015816
GCTAATT120700.010.4741431
GAGTAGC125700.010.0574864
CGTCGAG32800.09.53000451
CCTAAAT111900.09.4976411
GTAGCTG225000.09.4469156
GATATAT92350.09.4021581
Produced by FastQC (version 0.11.5)