FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19712313
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC261400.017.8478644
CCAAAGT318250.016.9516332
AAGTGCT304500.015.5042925
CTGTCTA135050.015.446324145
CAAAGTG334550.015.0241593
CCCAAAG415050.014.8489681
GCTGGGA586700.013.1840539
CTACTCG47450.012.584072
AGTGCTG419850.011.9230656
CCTATAT67950.011.2480251
TAGCTGG196400.011.0296897
GCTACTC129150.010.9751081
CGAGTAG45250.010.7396983
AGTAGCT161350.010.2409045
GCTATAT64750.010.0869791
TACTCGG57300.010.0562523
GCTATTA73600.010.0069361
GCTATAA80400.09.8519421
CCTATTA71750.09.6839191
GAGTAGC119150.09.6141844
Produced by FastQC (version 0.11.5)