FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815690
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC269700.017.6237284
CCAAAGT324350.016.005362
AAGTGCT314450.015.27125
CAAAGTG341300.014.9040763
CCCAAAG431600.014.3838311
CTGTCTA135700.014.198863145
GCTGGGA597350.013.0763119
GCTACTC125650.012.3380611
CGAGTAG43900.012.1886753
CTACTCG50850.012.1634792
AGTGCTG424650.011.7499696
TAGCTGG199350.011.7479037
AGTAGCT158200.011.5530325
GCTAATT117900.011.0853291
GCTATAT64150.010.8369711
TACTCGG62650.010.3155433
CCGAGTA55300.010.3049882
CCTATAT68500.09.9458071
CTACTTG117500.09.5224082
GCTATAC49000.09.5056821
Produced by FastQC (version 0.11.5)