FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20036899
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC265100.017.620784
CCAAAGT324750.016.5461942
ACGTCGA25950.015.2714431
CCCAAAG426800.015.1170191
CAAAGTG348150.014.9349973
AAGTGCT319900.014.8632565
CGTCGAG33450.013.32
CTACTCG47500.012.7319062
GCTGGGA603350.012.5459829
CCTATAT72200.011.651741
AGTGCTG427900.011.5985066
GCTAATT119900.011.0753331
TAGCTGG202900.010.9625577
AGTAGCT168200.010.8279795
GCTACTC129600.010.7292061
TACTCGG59750.010.7033533
CGAGTAG50100.010.5449813
GCTATAT64500.010.0245711
GCTATAG66950.09.5538811
CCGAGTA61250.09.3062682
Produced by FastQC (version 0.11.5)